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V. R. S. S. Mokkapati: Is it possible to sequence DNA/RNA through conventional DNA electrophoresis?

posted 27 Jul 2015, 01:06 by info admin
V.R.S.S. Mokkapati 1, Volkan Ozguz 2, Hikmet Budak 3
1,2 Nanotechnology research and Application Center (SUNUM), Sabanci Unviersity, Istanbul 34956, Turkey
3 Molecular Biology, Genetics & Bioengineering, Sabanci Unviersity, Istanbul 34956, Turkey

In this abstract we would like to propose and understand the feasibility of separation and sequencing of short DNA/RNA strands using conventional electrophoresis technique. Agarose/Polyacrylamide gels are integrated with graphene (mono/bi layers) containing nanopores or nanopores are generated after integration of graphene with the gels. During electrophoresis short strands of DNA/RNA samples that separate and pass through the nanochannels of the gel will then be directed towards graphene nanopores further detecting the change in the ionic current as they pass through these nanopores. Detection will be carried out with the help of the sensors crafted from graphene as shown in the figure.

VRSS Mokkapati is currently an FP7- Cofund Marie Curie fellow at Nanotechnology Research and Application Center, Sabanci University, Istanbul, Turkey. His current work involves in developing graphene integrated membranes for ultrafiltration of body fluids and Graphene for Transmission Electron Microscopy. He received his phd from Delft University of Technology, The Netherlands, where he worked on micro and nanofluidic devices for single cell and DNA analysis. Prior to joining Sabanci University he spent 2 years as a research fellow at Austrian Institute of Technology, Vienna, Austria, and National University of Singapore, Singapore, where he was working on disposable microfluidic devices and graphene for bio-medical applications and TEM respectively.