Yu.V. Stebunov 1, A.V. Arsenin 1, V.S. Volkov 1,2
1. Moscow Institute of Physics and Technology, Laboratory of Nanooptics and Plasmonics, Dolgoprudny, Russian Federation
2. University of Southern Denmark, Institute of Technology and Innovation, Odense, Denmark
Here, we describe a novel type of graphene oxide linking layer for highly sensitive biosensing based on surface plasmon resonance (SPR), which has become an indispesible tool for scientific research and drug development . During the last three decades, researchers have used only two technologies of linking layers for SPR biosensors, which are based on self-assembled monolayers of thiol molecules and on hydrogel layers. Using graphene and its derivatives, we developed biosensor chips for existing commercial biosensors, whose sensitivity is higher than for commercial sensor chips available on the market  (Fig. 1). Modification of carboxyl groups to N-hydroxysuccinimide esters in the flow cell of SPR biosensor demonstrated that the num-ber of carboxyl groups, which can be used for molecule immobilization, is more than 20 times higher in the graphene oxide linking layer than in the linking layer of commercial hydrogel-based sensor chip. In addition, the graphene oxide sensor chip was demonstrated to be 3 times more sensitive comparing to the commercial hydrogel chips when using in the standard biosensing protocol based on streptavidin-biotin interaction with streptavi-din immobilized on the GO surface via pi-stacking. This will enable us to investigate interactions of protein targets with small ligands and will broaden and accelerate academic and pharmaceutical research.
 Stebunov, Yu.V., Aftenieva, O.A., Arsenin, A.V., and Volkov, V.S. (2015), Highly sensitive and selective sensor chips with graphene-oxide linking layer. ACS Applied Materials and Interfaces., 7(39): 21727–21734.
 A.V. Arsenin, Yu.V. Stebunov. RU Pa-tent Application No. 2527699 (Feb 2013); US Patent Application No. 20150301039 (Oct 2015).
Yury Stebunov is currently a Research Fellow in the Laboratiry of Nanoopticcs and Plasmonics at the Moscow Institute of Physics and Technology (Russian Federation). He is primarily interested in the applications of graphene materials in biosensing. He received his B.S. and M.S. degrees from Florida International University and spent 2 years as a laboratory assistant (2011-2013) at the BiOptix Nanoprom (subsidiary of BiOptix Diagnostics, Inc, USA), Moscow, Russia.
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