Ivan Komarov: Reduced graphene oxide based biosensors on flexible substrate

Komarov I.A. 1 , Rubtsova E.V. 1 , Smagulova S.A. 2 , Alexandrov G.N. 2 , Golovin A.V. 3 , Lapashina A. 3 1 National Research University of Electronic Technology, Moscow, Russian Federation 2 North-Eastern Federal University, Yakutsk, Russian Federation 3 Lomonosov Moscow State University, Moscow, Russian Federation Graphene and its derivatives (especially graphene oxide) are very perspective materials that attend great interest from scientists from over the world. Graphene oxide have some advantages over CVD or mechanically exfoliated graphene in context of deposition on flexible substrate. Reduced graphene oxide is a perspective material for flexible electronic, especially in sphere of personal health monitoring.  On the other side, there is a challenge to develop highly sensitive sensors for detection of disease agents in blood. One of most promising materials for highly selective detection of  disease agents are aptamers [1-3] which are small artificial DNA parts, that can selectively couple with target agent.  In this work, we develop design of flexible reduced graphene oxide based sensor on flexible PET substrate. We also develop method for covalent coupling of thrombin aptamers to the surface of reduced graphene oxide. We made experiments with thrombin and albumin exposition on the surface of sensor. In these experiments, we showed significant difference between sensors exposed with thrombin and albumin. We observed linear law resistance increase from 1,04% to 3,08% with increase of thrombin concentration (from 0,1 µM to 10 µM). Thus we developed new design of reduced graphene oxide based flexible sensor and find out its response on thrombin and response dependence on concentration.   1. Hye-Mi So, Keehoon Won, Yong Hwan Kim, Byoung-Kye Kim, Beyong Hwan Ryu, Pil Sun Na, Hyojin Kim, and Jeong-O Lee. J. AM. CHEM. SOC (2005) 127, 11906. 2. Taechang An, Ki Su Kim,b Sei Kwang Hahn, Geunbae Lim. Lab Chip (2010) 10, 2052 3. Wang C, Kim J, Zhu Y, Yang J, Lee GH, Lee S, Yu J, Pei R, Liu G, Nuckolls C, Hone J, Lin Q. Biosensors and Bioelectronics (2015) 71,  222.   Ivan Komarov is currently a leading engineer in National Reserach University of Electronic Technology. His main scientific interests are carbon nanomaterials, flexible and organic electronic and sensors. He received his M.S. degree in Engineering and Technology (2010) and a Ph.D. in Technical sciences (2013) from from National Reserach University of Electronic Technology where he studied the electrical and sensing properties of carbon nanotubes. During finishing his Ph.D, he spent 6 months as a trainee in the Nokia Research center at Skolkovo Innovation Center studying organic and flexible electronic. After that he returned to the National Reserach University of Electronic Technology to study biological sensing with carbon nanomaterials.